By Tong-Cun Zhang, Motowo Nakajima
At the ICAB 2014, researchers from around the globe will assemble to debate the newest medical examine, findings and applied sciences relating Microbial Genetics and Breeding, Optimization and regulate of organic procedures, organic Separation and organic Purification, and Advances in Biotechnology.
This convention will offer a platform for tutorial alternate at the software of biotechnology among family and foreign universities, learn institutes, company specialists and students. The members will concentrate on the overseas improvement and destiny traits. the development will lay an excellent starting place for addressing key technical demanding situations in a number of components of utilized biotechnology, delivering possibilities to advertise the advance and enlargement of the biotechnology industry.
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Extra info for Advances in Applied Biotechnology: Proceedings of the 2nd International Conference on Applied Biotechnology (ICAB 2014)-Volume II
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Identiﬁcation of Glu-198 as an active site carboxylate group with acid catalytic function. Arch Biochem Biophys 315: 323–330 5. Keresztessy Z, Brown K, Dunn MA, Hughes MA (2001) Identiﬁcation of essential active-site residues in the cyanogenic β-glucosidase (linamarase) from cassava (Manihot esculenta Crantz) by site-directed mutagenesis. Biochem J 353:199–205 6. Peifeng S, Lili F, Xuepiao S, Jiaming Z (2012) Cloning, expression of linamarase in cassava and characterization of its recombinant protein activity.
183 U/mL. 084 U/mL. Recombinant strain containing pLeuDHFDH used for Fig. 3 The enzyme activity of LeuDH (a) and FDH (b) of recombinant strains of E. coli BL21 (DE3) Fig. 4 Concentration of the L-tert-Leucine in the reaction system using different recombinant strains and conditions. 1 Whole cells of the stains containing pLeuDH and pFDH with NAD; 2 whole cells of the strains containing pLeuDH and pFDH without NAD; 3 cell extraction of the strains containing pLeuDH and pFDH with NAD; 4 whole cells of the strains containing pLeuDHFDH with NAD; 5 whole cells of the strain containing pLeuDHFDH without NAD; 6 cell extraction of the strains containing pLeuDHFDH with NAD 3 Construction of L-tert-Leucine Producing Strain … 27 simultaneous expression of LeuDH and FDH was also constructed.