Animal Cell Technology: Basic & Applied Aspects: Proceedings by R. E. Spier (auth.), H. Murakami, S. Shirahata, H. Tachibana

By R. E. Spier (auth.), H. Murakami, S. Shirahata, H. Tachibana (eds.)

New information on animal telephone expertise are introduced jointly during this quantity, with emphasis given to the fundamental characterization of phone traces. The benefits of alternative cellphone tradition structures are tested and investigations into the standards influencing mobile progress and productiveness are offered. a unique part bargains with the organic houses of proteins produced through engineered animal cells. All these interested in the tradition of animal cells will locate this quantity worthwhile.

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Extra info for Animal Cell Technology: Basic & Applied Aspects: Proceedings of the Fourth Annual Meeting of the Japanese Association for Animal Cell Technology, Fukuoka, Japan, 13–15 November 1991

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HIV-l, T4 phage and A phage were assayed by plaque formig method. DHBV was assayed by 50% duck infectivity. ). AI I proteins were guaranteed grade. The concentrations of proteins were determined by proteindye binding assay using CBB-G250. O). The concentrations of ONAs were electrophoresed and detected by ethidium bromide staining. (S)Ultrafi Itration; The dead-end type filtration was carried out under the constant transmembrane pressure (TMP) of 200mmHg at 20·C with the filtration volume of 311m 2 • The change in filtration rate was I imited to within 50% of the initial one.

In the case of virus removal using BMM, the 1inear1ity (i. e. (7) When the virus particle is represented by monodispersed particles, then the value of -10g1~ (virus concentration in filtrate) or ~ value, increases 1inear~y with filtration step (repeating times). The sindbis virus and Japanese encephalitis virus (JEV) are examples holding the above linearity. The double filtration is very effective in removing these viruses. That is, the additive low in ~ value for virus removability in multistep filtration holds.

4 Assay of ~ nitroblue tetrazolium (NBT) reduction activity. A NBT reduction activity of U-M and U-937 cells , which indicates active oxygen (0 2-, H20 2 ) production was determined in the presence or absence of lectins by tnesnethods of Koizumi et al(3). Briefly, cells at the density of 2 x 10 cells/ml were suspended in 200 ul of the culture medium containing NBT reagent, 100 ng TPA and 10 ug/ml of each lectin. The reaction mixture was incubated at 37 °c for 2 hr and cells were collected by centrifugation at 1,000 x g for 10 min.

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