Applied biochemistry and bioengineering by Ichiro Chibata, Lemuel B. Wingard

By Ichiro Chibata, Lemuel B. Wingard

Immobilized Microbial Cells, quantity four presents an summary of the tools of immobilization, functions, and methods of using immobilized microbial cells and subcellular organelles and chloroplasts as biocatalysts.

This quantity is made out of seven chapters. It starts off with the historic historical past of immobilized mobile learn. next chapters concentrate on the equipment of immobilization and purposes of immobilized microbial cells, dwelling cells, and organelles. The final chapters talk about fuel construction of immobilized cells for strength new release and the chemical engineering research of immobilized-cell systems.

The booklet can be of serious use to chemists and chemical engineers.

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Technol. 4, 93. Cho, G. , and Choi, C. Y. (1981). Biotechnol. Lett. 3, 667. Chua, I. , and Taguchi, H. /. Ferment. Technol. 58, 123. , and Hebeault, J. M. (1979). Cell. Immobilisees Colloq. 1979 pp. 211-217. Constantinides, A. (1980). Biotechnol. Bioeng. 22, 119. , and Vieth, W. K. (1981). Biotechnol Bioeng. 23(4), 8 9 9 916. , Navarro, J . , and Moll, M. (1976). Proc. Int. Ferment. , 5th, p. 298. , and Baratti, J. (1980). Biotechnol Bioeng. 22, 1155. Dangulis, A. , Brown, N. , Cluett, W. , and Dunlop, D.

T. (1982). Enzyme Microb. Technol. 4, 93. Cho, G. , and Choi, C. Y. (1981). Biotechnol. Lett. 3, 667. Chua, I. , and Taguchi, H. /. Ferment. Technol. 58, 123. , and Hebeault, J. M. (1979). Cell. Immobilisees Colloq. 1979 pp. 211-217. Constantinides, A. (1980). Biotechnol. Bioeng. 22, 119. , and Vieth, W. K. (1981). Biotechnol Bioeng. 23(4), 8 9 9 916. , Navarro, J . , and Moll, M. (1976). Proc. Int. Ferment. , 5th, p. 298. , and Baratti, J. (1980). Biotechnol Bioeng. 22, 1155. Dangulis, A. , Brown, N.

Thus diffusion limitations are to be expected. 5. Cell loading capacity can be rather high, and values up to 70% (£. colt) or 30% (bakers' yeast) on a wet-weight basis can well be obtained. Shrinking of the ionotropic gel by partial drying is the key step to obtaining such cell densities. 6. Yield of immobilized activity is moderate; 40% has been a typical value for penicillin acylase in E. coli cells (Eng, 1980). 7. Growth experiments of immobilized cells show the viability of E. coli and yeast cells (Eng, 1980; Klein and Kressdorf, 1982).

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